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青岛能源所基于“剪刀石头布”策略实现快速多轮基因编辑

撰稿: 青岛生物能源与过程研究所 发布时间:2020-12-11

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  图1. 基于“剪刀石头布”策略的多基因编辑方法示意图。A. 三个能够循环消除的基因编辑辅助质粒,在实施基因编辑的同时能消除上一轮的质粒。 B. 相较于传统方法,本方法不需要单独的筛选标记或质粒消除步骤。

   

  图2. 基于“剪刀石头布”策略的多轮基因编辑流程图。A. “剪刀石头布”基因编辑辅助质粒构建流程图。B. 连续多轮基因编辑步骤流程图。

  本研究相关质粒可从第三方开放获取:MolecularCloud ()MC_0101139, MC_0101140MC_0101141。建议同时获取MC_0000011以及MC_0000012

  原文链接:  

  Jichao Wang, Xinyue Sui, Yamei Ding, Yingxin Fu, Xinjun Feng, Min Liu, Youming Zhang, Mo Xian, Guang Zhao, A fast and robust iterative genome-editing method based on a Rock-Paper-Scissors strategy, Nucleic Acids Research, 2020, DOI: 10.1093/nar/gkaa1141